Objectives: Assess the knowledge and attitude on prevention of Needle Stick Injuries among students. Evaluate the effectiveness of structured teaching programme on knowledge and attitude regarding prevention of Needle Stick Injuries. Determine the association between knowledge and attitude scores of nursing students on prevention of Needle Stick Injuries. Methods: The study was conducted in a selected nursing college at Coimbatore and the research method adopted was quasi experimental, time series design. As per the inclusion criteria 185 samples were selected. The pre-test score of knowledge and attitude was assessed by the structured questionnaire. A structured teaching program on prevention of Needle Stick Injuries (duration 30-45 minutes) was given and the post-test assessment was done on the 8th day using the same tool. Post-test-II was done on the 22nd day using the same tool. Results: The study revealed an increase in the knowledge and attitude level following the structured teaching program. In this study the statistical analysis showed that there was a significant improvement (t=1.64, p< 0.05), in the level of knowledge and attitude after implementation of structured teaching programme and significant association (χ2=3.84, p<0.05) between pre-test score of knowledge and attitude on prevention of Needle Stick Injuries with gender, year of studying, past history of Needle Stick Injuries. Conclusion: Structured Teaching Programme was helpful in improving knowledge and attitude among students regarding prevention of Needle Stick Injuries.